A 5-fold dilution of plasma is assayed spectrophotometrically for cholesterol - concentration of diluted plasma

Question

A 5-fold dilution of plasma is assayed spectrophotometrically for cholesterol.

Given the following spectrophotometic data, what is the concentration of cholesterol in the diluted plasma in micromol/mL? (DO NOT INCLUDE THE UNITS IN YOUR ANSWER)

A₅₀₀ standard = 0.59
A₅₀₀ sample = 0.41
C (standard) = 4.2mM

Answer 1

Foumula for concentration of cholesterol for each sample: .

Substitute , and .

Concentration of each sample is 2.9186 mM.

5-fold dilution means that a solution is diluted until its molarity is one-fifth of its original value, that is, the volume of the solution is five times its original value.

Concentration of cholesterol in the diluted plasma is .

Comments

Answer is wrong.